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pdf 15 DOWNLOADNecrotizing enterocolitis in an immunocompetent patient with Chlamydia trachomatis infection. Necrotizing enterocolitis (NEC) is a devastating disease of the intestinal tract in neonates. The incidence of NEC in immunocompromised neonates is generally higher than in the general population. We report an unusual case of neonatal NEC in an immunocompetent neonate that had Chlamydia trachomatis isolated from his stool.Characterization of fibroblast-derived pro-collagen I gene transcription in normal and keloid fibroblasts. Collagen is the major extracellular matrix protein of fibroblasts. We have developed a transient expression assay to determine the relative transcription rates of two fibroblast-derived pro-collagen I mRNA species. Normal human adult fibroblasts (NHF) and keloid fibroblasts (KF) were infected with a recombinant retrovirus containing the chloramphenicol acetyl transferase (CAT) gene linked to a 5'-flanking fragment of the collagen type I promoter and were grown in the presence of 5-bromo-2'-deoxyuridine. CAT-mRNA levels were assayed in cell extracts with a CAT-specific monoclonal antibody. Infection of NHF or KF cells with the CAT-recombinant retrovirus resulted in a 4- to 5-fold increase in CAT-mRNA levels over the control, uninfected cells. Subsequent addition of TPA increased the CAT-mRNA levels 3- to 5-fold. Transcription rates of pro-collagen I mRNA increased linearly over a 6-hr period when the infection was followed by a 6-hr treatment with TPA. The transcription rates of these two pro-collagen I mRNA species were identical in NHF and KF cells. Our data suggest that collagen synthesis in NHF is controlled at the level of transcription.Campeonato Paraguay: Gamba’s Landa handed two-match ban Paraguayan side Gamba have handed coach Pedro Landa a two-match ban after his action in the team’s recent 7-0 Copa Sudamericana semi-final first-leg loss to Argentine outfit San Lorenzo. The 35-year-old is likely to return to the San Lorenzo side in the second

 

 


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40 Hadis Penawar Hati Pdf Download
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